her2 breast cancer cell lines skbr3  (DSMZ)

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: A coiled coil-based pre-targeting drug delivery system for precise treatment of breast cancer.

doi: 10.1016/j.ejpb.2025.114794

Figure Lengend Snippet: Fig. 4. Visualization of the expression levels and binding patterns of EGFR, Her2 and TROP2 in breast cancer tissues, as well as the specificity of four ADCs targeting these proteins. (a) Microphotographs of mIF of EGFR, Her2 and TROP2 in breast cancer tissues along with corresponding 100x sample regions for each marker. (b) Heatmap shows the expression pattern of EGFR, Her2 and TROP2 in breast cancer tissues. (c) The boxplot represents the average percentages of each marker. (d) Microphotographs of mIF of binding specificities of four ADCs in breast cancer tissues along with corresponding 100x sample regions for each ADC. (e) The per centage of each ADC is illustrated in heatmap. (f) The average percentage of each ADC binding is shown in boxplot.

Article Snippet: TNBC cell lines MDA-MB-468 (ACC 738), MDA-MB231 (ACC 732), MDA-MB-453 (ACC 65) and Hs578T (ACC 781), ER+ breast cancer cell line MCF7 (ACC 115), and Her2+ breast cancer cell lines SKBR3 (ACC 736) and BT474 (ACC 64) were purchased from the Leibniz Institute DSMZ.

Techniques: Expressing, Binding Assay, Marker

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: A coiled coil-based pre-targeting drug delivery system for precise treatment of breast cancer.

doi: 10.1016/j.ejpb.2025.114794

Figure Lengend Snippet: Fig. 5. Specific cytotoxicity of pre-targeting complex. (a) Breast cancer cell lines were treated with increasing concentration (10, 20, 40, 80, 160, 320, 640 nM) of scFv-Zip2-Zip1-MMAE, scFv-Erbitux-MMAE, scFv-Sacit-MMAE, combination-Zip1-MMAE, scFv-Zip2-Zip1-SNAP, scFv-SNAP, combination-Zip1-SNAP, Zip1-MMAE or MMAE at 37 ◦C for 72 h, cell lines except SKBR3 were treated with increasing concentration (1.25, 2.5, 5, 10, 20, 40, 80, 160, 320, 640 nM) of scFv-Herceptin- MMAE. (b) SKBR3 was incubated with increasing concentration (0.03125, 0.0625, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16 nM) of scFv-Herceptin-MMAE at 37 ◦C for 72 h. Cell viability was determined by XTT) Kit II, and the experiment was carried out in triplicates for three times. One independent experiment was shown with the mean ± SD.

Article Snippet: TNBC cell lines MDA-MB-468 (ACC 738), MDA-MB231 (ACC 732), MDA-MB-453 (ACC 65) and Hs578T (ACC 781), ER+ breast cancer cell line MCF7 (ACC 115), and Her2+ breast cancer cell lines SKBR3 (ACC 736) and BT474 (ACC 64) were purchased from the Leibniz Institute DSMZ.

Techniques: Concentration Assay, Incubation

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: A coiled coil-based pre-targeting drug delivery system for precise treatment of breast cancer.

doi: 10.1016/j.ejpb.2025.114794

Figure Lengend Snippet: Fig. 6. Induction of apoptosis by Trop2-targeting ADCs. (a) Scatter plot of one measurement as an example. (b) Statistic analysis of apoptotic cells including early (Q3) and late (Q2) apoptotic cells inducted by Trop2-, EGFR- and Her2 targeting ADCs. The experiment was carried out independently in triplicates for three times. Data were shown as mean ± SEM. The statistical analysis was carried out using one-way ANOVA and corrected by Tukey’s test (*p＜0.05, **** p＜0.0001).

Article Snippet: TNBC cell lines MDA-MB-468 (ACC 738), MDA-MB231 (ACC 732), MDA-MB-453 (ACC 65) and Hs578T (ACC 781), ER+ breast cancer cell line MCF7 (ACC 115), and Her2+ breast cancer cell lines SKBR3 (ACC 736) and BT474 (ACC 64) were purchased from the Leibniz Institute DSMZ.

Techniques: